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piezo1 mechanosensitive ion channels  (Novus Biologicals)


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    Novus Biologicals piezo1 mechanosensitive ion channels
    Heightened <t>piezo1</t> expression around the stiff implant. A) Representative images showing Piezo1 expression around the flexible (left) and stiff (right) implant site (*). B) Quantification of normalized intensity as a function of distance from the implant (μm). C) Normalized intensity averaged across the first 50 μm from the implant site. 19 images for the flexible and 22 images for the stiff group were analyzed. D) Representative images showing Piezo1 (cyan), Iba-1 (green), GFAP (red), and merged expression around a stiff implant, with the zoomed inset with DAPI (blue) overlay. E) Correlation of total piezo1 expression with total Iba-1 expression per image for flexible (left, blue) and stiff (right, red) groups. The slope is significant for the stiff group, indicating a strong correlation between Piezo1 expression and Iba-1 intensity. F) Correlation of total piezo1 expression with total GFAP expression per image for flexible (left, blue) and stiff (right, red). 4 animals were implanted in each group. C) Two-way ANOVA with Sidak’s multiple comparison test was used, E-F) Data was normalized per image and the mean set to 0. A simple linear regression was performed, dashed lines indicate 95 % confidence intervals, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The scale bar is 100 μm in A) and D) and 50 μm in the zoomed inset in D). C) Data are mean ± SEM. E-F) Data is sum.
    Piezo1 Mechanosensitive Ion Channels, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A comparative study assessing neural recording quality and inflammatory tissue response between stiff and flexible microelectrode arrays"

    Article Title: A comparative study assessing neural recording quality and inflammatory tissue response between stiff and flexible microelectrode arrays

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2025.123929

    Heightened piezo1 expression around the stiff implant. A) Representative images showing Piezo1 expression around the flexible (left) and stiff (right) implant site (*). B) Quantification of normalized intensity as a function of distance from the implant (μm). C) Normalized intensity averaged across the first 50 μm from the implant site. 19 images for the flexible and 22 images for the stiff group were analyzed. D) Representative images showing Piezo1 (cyan), Iba-1 (green), GFAP (red), and merged expression around a stiff implant, with the zoomed inset with DAPI (blue) overlay. E) Correlation of total piezo1 expression with total Iba-1 expression per image for flexible (left, blue) and stiff (right, red) groups. The slope is significant for the stiff group, indicating a strong correlation between Piezo1 expression and Iba-1 intensity. F) Correlation of total piezo1 expression with total GFAP expression per image for flexible (left, blue) and stiff (right, red). 4 animals were implanted in each group. C) Two-way ANOVA with Sidak’s multiple comparison test was used, E-F) Data was normalized per image and the mean set to 0. A simple linear regression was performed, dashed lines indicate 95 % confidence intervals, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The scale bar is 100 μm in A) and D) and 50 μm in the zoomed inset in D). C) Data are mean ± SEM. E-F) Data is sum.
    Figure Legend Snippet: Heightened piezo1 expression around the stiff implant. A) Representative images showing Piezo1 expression around the flexible (left) and stiff (right) implant site (*). B) Quantification of normalized intensity as a function of distance from the implant (μm). C) Normalized intensity averaged across the first 50 μm from the implant site. 19 images for the flexible and 22 images for the stiff group were analyzed. D) Representative images showing Piezo1 (cyan), Iba-1 (green), GFAP (red), and merged expression around a stiff implant, with the zoomed inset with DAPI (blue) overlay. E) Correlation of total piezo1 expression with total Iba-1 expression per image for flexible (left, blue) and stiff (right, red) groups. The slope is significant for the stiff group, indicating a strong correlation between Piezo1 expression and Iba-1 intensity. F) Correlation of total piezo1 expression with total GFAP expression per image for flexible (left, blue) and stiff (right, red). 4 animals were implanted in each group. C) Two-way ANOVA with Sidak’s multiple comparison test was used, E-F) Data was normalized per image and the mean set to 0. A simple linear regression was performed, dashed lines indicate 95 % confidence intervals, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The scale bar is 100 μm in A) and D) and 50 μm in the zoomed inset in D). C) Data are mean ± SEM. E-F) Data is sum.

    Techniques Used: Expressing, Comparison

    Cell-specific intensity analysis. A) Microglia mask of a sample image using Iba-1. Black portions represent the extracted cell structures. This mask was used to identify the sample microglia shown in B). B) Piezo1 (blue) stained image showing activated microglia expressing Piezo1. C) Average within-microglia Piezo1 intensity with distance from the probe center. D) Average within-microglia Iba-1 intensity with distance from the probe center. E) Astrocyte mask of the above image using GFAP. Black portions represent the extracted cell structures. This mask was used to identify the sample astrocyte processes shown in F). F) Piezo1 (blue) stained image showing astrocyte processes expressing Piezo1. G) Average within-astrocyte Piezo1 intensity with distance from the probe center. H) Average within-astrocyte GFAP intensity with distance from the probe center. For all microglia and astrocyte analysis, the increase in intensity with proximity to the injury is highly significant for both probe substrates, and the cells surrounding stiff probes have higher intensity expression than the flexible. I) Piezo1 (red) stained image overlayed with NeuN ROIs produced by Cellpose. J) Piezo1 (red) stained image merged with NeuN (green). K) Average within-neuron Piezo1 intensity with distance from the probe center. Sample DAPI (white) stained image for a L) stiff and M) flexible probe implant. N) Average DAPI circularity index for all images analyzed above. Bar plot is the same data, but binned into two large bins rather than 10 evenly spaced bins. For A) and E), DAPI is overlayed on the mask. DAPI that passes through masked cell structures is seen as red, while DAPI covered by the mask is seen as yellow. Scale bar is 100 μm and 50 μm in zoomed insets in M&N. * Indicates implantation site. For comparisons between stiff and flexible, a two-way ANOVA with Sidak’s multiple comparison test was used. For correlation of intensity with distance from the probe, a Spearman’s r correlation test was used due to the non-normality of many of the datasets, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data is mean ± SEM.
    Figure Legend Snippet: Cell-specific intensity analysis. A) Microglia mask of a sample image using Iba-1. Black portions represent the extracted cell structures. This mask was used to identify the sample microglia shown in B). B) Piezo1 (blue) stained image showing activated microglia expressing Piezo1. C) Average within-microglia Piezo1 intensity with distance from the probe center. D) Average within-microglia Iba-1 intensity with distance from the probe center. E) Astrocyte mask of the above image using GFAP. Black portions represent the extracted cell structures. This mask was used to identify the sample astrocyte processes shown in F). F) Piezo1 (blue) stained image showing astrocyte processes expressing Piezo1. G) Average within-astrocyte Piezo1 intensity with distance from the probe center. H) Average within-astrocyte GFAP intensity with distance from the probe center. For all microglia and astrocyte analysis, the increase in intensity with proximity to the injury is highly significant for both probe substrates, and the cells surrounding stiff probes have higher intensity expression than the flexible. I) Piezo1 (red) stained image overlayed with NeuN ROIs produced by Cellpose. J) Piezo1 (red) stained image merged with NeuN (green). K) Average within-neuron Piezo1 intensity with distance from the probe center. Sample DAPI (white) stained image for a L) stiff and M) flexible probe implant. N) Average DAPI circularity index for all images analyzed above. Bar plot is the same data, but binned into two large bins rather than 10 evenly spaced bins. For A) and E), DAPI is overlayed on the mask. DAPI that passes through masked cell structures is seen as red, while DAPI covered by the mask is seen as yellow. Scale bar is 100 μm and 50 μm in zoomed insets in M&N. * Indicates implantation site. For comparisons between stiff and flexible, a two-way ANOVA with Sidak’s multiple comparison test was used. For correlation of intensity with distance from the probe, a Spearman’s r correlation test was used due to the non-normality of many of the datasets, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data is mean ± SEM.

    Techniques Used: Staining, Expressing, Produced, Comparison



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    (A) Representative tracing of contractile response to cumulative additions of the <t>Piezo1</t> agonist Yoda1 to thoracic aorta ring with PVAT. Piezo1 agonists did not cause a concentration dependent contraction. Quantification of multiple experiments using Yoda1 (B) and Jedi2 (C) as agonists (grey) vs Vehicle (white, same vehicle for B and C). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).
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    Image Search Results


    Heightened piezo1 expression around the stiff implant. A) Representative images showing Piezo1 expression around the flexible (left) and stiff (right) implant site (*). B) Quantification of normalized intensity as a function of distance from the implant (μm). C) Normalized intensity averaged across the first 50 μm from the implant site. 19 images for the flexible and 22 images for the stiff group were analyzed. D) Representative images showing Piezo1 (cyan), Iba-1 (green), GFAP (red), and merged expression around a stiff implant, with the zoomed inset with DAPI (blue) overlay. E) Correlation of total piezo1 expression with total Iba-1 expression per image for flexible (left, blue) and stiff (right, red) groups. The slope is significant for the stiff group, indicating a strong correlation between Piezo1 expression and Iba-1 intensity. F) Correlation of total piezo1 expression with total GFAP expression per image for flexible (left, blue) and stiff (right, red). 4 animals were implanted in each group. C) Two-way ANOVA with Sidak’s multiple comparison test was used, E-F) Data was normalized per image and the mean set to 0. A simple linear regression was performed, dashed lines indicate 95 % confidence intervals, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The scale bar is 100 μm in A) and D) and 50 μm in the zoomed inset in D). C) Data are mean ± SEM. E-F) Data is sum.

    Journal: Biomaterials

    Article Title: A comparative study assessing neural recording quality and inflammatory tissue response between stiff and flexible microelectrode arrays

    doi: 10.1016/j.biomaterials.2025.123929

    Figure Lengend Snippet: Heightened piezo1 expression around the stiff implant. A) Representative images showing Piezo1 expression around the flexible (left) and stiff (right) implant site (*). B) Quantification of normalized intensity as a function of distance from the implant (μm). C) Normalized intensity averaged across the first 50 μm from the implant site. 19 images for the flexible and 22 images for the stiff group were analyzed. D) Representative images showing Piezo1 (cyan), Iba-1 (green), GFAP (red), and merged expression around a stiff implant, with the zoomed inset with DAPI (blue) overlay. E) Correlation of total piezo1 expression with total Iba-1 expression per image for flexible (left, blue) and stiff (right, red) groups. The slope is significant for the stiff group, indicating a strong correlation between Piezo1 expression and Iba-1 intensity. F) Correlation of total piezo1 expression with total GFAP expression per image for flexible (left, blue) and stiff (right, red). 4 animals were implanted in each group. C) Two-way ANOVA with Sidak’s multiple comparison test was used, E-F) Data was normalized per image and the mean set to 0. A simple linear regression was performed, dashed lines indicate 95 % confidence intervals, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The scale bar is 100 μm in A) and D) and 50 μm in the zoomed inset in D). C) Data are mean ± SEM. E-F) Data is sum.

    Article Snippet: Antibodies to visualize astrocytes (GFAP, 1:500, Z033401 Dako), macrophage/microglia IBA-1(microglia, 1:500, NC9288364 Fisher), Piezo1 mechanosensitive ion channels (Piezo1,1:100, NBP275617 Novus Biologicals), neuronal nuclei (NeuN, 1:750, CAD69 Abcam), and neural filament (NF200, 1:250 MAB5256 Millipore) were used.

    Techniques: Expressing, Comparison

    Cell-specific intensity analysis. A) Microglia mask of a sample image using Iba-1. Black portions represent the extracted cell structures. This mask was used to identify the sample microglia shown in B). B) Piezo1 (blue) stained image showing activated microglia expressing Piezo1. C) Average within-microglia Piezo1 intensity with distance from the probe center. D) Average within-microglia Iba-1 intensity with distance from the probe center. E) Astrocyte mask of the above image using GFAP. Black portions represent the extracted cell structures. This mask was used to identify the sample astrocyte processes shown in F). F) Piezo1 (blue) stained image showing astrocyte processes expressing Piezo1. G) Average within-astrocyte Piezo1 intensity with distance from the probe center. H) Average within-astrocyte GFAP intensity with distance from the probe center. For all microglia and astrocyte analysis, the increase in intensity with proximity to the injury is highly significant for both probe substrates, and the cells surrounding stiff probes have higher intensity expression than the flexible. I) Piezo1 (red) stained image overlayed with NeuN ROIs produced by Cellpose. J) Piezo1 (red) stained image merged with NeuN (green). K) Average within-neuron Piezo1 intensity with distance from the probe center. Sample DAPI (white) stained image for a L) stiff and M) flexible probe implant. N) Average DAPI circularity index for all images analyzed above. Bar plot is the same data, but binned into two large bins rather than 10 evenly spaced bins. For A) and E), DAPI is overlayed on the mask. DAPI that passes through masked cell structures is seen as red, while DAPI covered by the mask is seen as yellow. Scale bar is 100 μm and 50 μm in zoomed insets in M&N. * Indicates implantation site. For comparisons between stiff and flexible, a two-way ANOVA with Sidak’s multiple comparison test was used. For correlation of intensity with distance from the probe, a Spearman’s r correlation test was used due to the non-normality of many of the datasets, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data is mean ± SEM.

    Journal: Biomaterials

    Article Title: A comparative study assessing neural recording quality and inflammatory tissue response between stiff and flexible microelectrode arrays

    doi: 10.1016/j.biomaterials.2025.123929

    Figure Lengend Snippet: Cell-specific intensity analysis. A) Microglia mask of a sample image using Iba-1. Black portions represent the extracted cell structures. This mask was used to identify the sample microglia shown in B). B) Piezo1 (blue) stained image showing activated microglia expressing Piezo1. C) Average within-microglia Piezo1 intensity with distance from the probe center. D) Average within-microglia Iba-1 intensity with distance from the probe center. E) Astrocyte mask of the above image using GFAP. Black portions represent the extracted cell structures. This mask was used to identify the sample astrocyte processes shown in F). F) Piezo1 (blue) stained image showing astrocyte processes expressing Piezo1. G) Average within-astrocyte Piezo1 intensity with distance from the probe center. H) Average within-astrocyte GFAP intensity with distance from the probe center. For all microglia and astrocyte analysis, the increase in intensity with proximity to the injury is highly significant for both probe substrates, and the cells surrounding stiff probes have higher intensity expression than the flexible. I) Piezo1 (red) stained image overlayed with NeuN ROIs produced by Cellpose. J) Piezo1 (red) stained image merged with NeuN (green). K) Average within-neuron Piezo1 intensity with distance from the probe center. Sample DAPI (white) stained image for a L) stiff and M) flexible probe implant. N) Average DAPI circularity index for all images analyzed above. Bar plot is the same data, but binned into two large bins rather than 10 evenly spaced bins. For A) and E), DAPI is overlayed on the mask. DAPI that passes through masked cell structures is seen as red, while DAPI covered by the mask is seen as yellow. Scale bar is 100 μm and 50 μm in zoomed insets in M&N. * Indicates implantation site. For comparisons between stiff and flexible, a two-way ANOVA with Sidak’s multiple comparison test was used. For correlation of intensity with distance from the probe, a Spearman’s r correlation test was used due to the non-normality of many of the datasets, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data is mean ± SEM.

    Article Snippet: Antibodies to visualize astrocytes (GFAP, 1:500, Z033401 Dako), macrophage/microglia IBA-1(microglia, 1:500, NC9288364 Fisher), Piezo1 mechanosensitive ion channels (Piezo1,1:100, NBP275617 Novus Biologicals), neuronal nuclei (NeuN, 1:750, CAD69 Abcam), and neural filament (NF200, 1:250 MAB5256 Millipore) were used.

    Techniques: Staining, Expressing, Produced, Comparison

    (A) Representative tracing of contractile response to cumulative additions of the Piezo1 agonist Yoda1 to thoracic aorta ring with PVAT. Piezo1 agonists did not cause a concentration dependent contraction. Quantification of multiple experiments using Yoda1 (B) and Jedi2 (C) as agonists (grey) vs Vehicle (white, same vehicle for B and C). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: (A) Representative tracing of contractile response to cumulative additions of the Piezo1 agonist Yoda1 to thoracic aorta ring with PVAT. Piezo1 agonists did not cause a concentration dependent contraction. Quantification of multiple experiments using Yoda1 (B) and Jedi2 (C) as agonists (grey) vs Vehicle (white, same vehicle for B and C). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques: Concentration Assay

    (A) Representative tracing of relaxant response to cumulative additions of the Piezo1 agonist Yoda1 in half-maximally PE contracted thoracic aorta ring with PVAT. Neither the Piezo1 agonist Yoda1 (B) nor Jedi2 (C) (grey) caused concentration dependent relaxation vs Vehicle (black). (A) Arrows indicate each cumulative step. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: (A) Representative tracing of relaxant response to cumulative additions of the Piezo1 agonist Yoda1 in half-maximally PE contracted thoracic aorta ring with PVAT. Neither the Piezo1 agonist Yoda1 (B) nor Jedi2 (C) (grey) caused concentration dependent relaxation vs Vehicle (black). (A) Arrows indicate each cumulative step. (B, C) Symbols represent mean±SEM for N reported, −P = without PVAT (open) +P = with PVAT (filled).

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques: Concentration Assay

    Piezo1 mRNA is present in vessels and PVAT of the male Sprague Dawley rat aorta (A) and mesenteric vessel (B) as well as in the adipocytes and SVF of the mesenteric PVAT(C). Piezo1, Piezo2, TRPV4, TMEM16, and Panx1 mRNA were measured using RT-PCR and beta actin as a calibrator control. Bars are mean+SEM with scattered individual points for an N = 4–5. Stat test was a one-way ANOVA, *p < 0.05.

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: Piezo1 mRNA is present in vessels and PVAT of the male Sprague Dawley rat aorta (A) and mesenteric vessel (B) as well as in the adipocytes and SVF of the mesenteric PVAT(C). Piezo1, Piezo2, TRPV4, TMEM16, and Panx1 mRNA were measured using RT-PCR and beta actin as a calibrator control. Bars are mean+SEM with scattered individual points for an N = 4–5. Stat test was a one-way ANOVA, *p < 0.05.

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Control

    Detection of Piezo1 in distinct layers (endothelium [E], media [M], and PVAT [P]) of isolated rat thoracic aorta (A), superior mesenteric artery (B), mesenteric resistance vessels (artery [A] and vein [V]) (C), and positive control rat kidney (D). L = lumen. Top panel for each set of images shows DAPI as a nuclei marker and FITC indicating Piezo1 signal, while bottom panel shows tissue without primary antibody (no 1°). White arrows indicate staining of interest. Images are representative of N = 5 taken at 20x with 100 μm scale bars bottom right.

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: Detection of Piezo1 in distinct layers (endothelium [E], media [M], and PVAT [P]) of isolated rat thoracic aorta (A), superior mesenteric artery (B), mesenteric resistance vessels (artery [A] and vein [V]) (C), and positive control rat kidney (D). L = lumen. Top panel for each set of images shows DAPI as a nuclei marker and FITC indicating Piezo1 signal, while bottom panel shows tissue without primary antibody (no 1°). White arrows indicate staining of interest. Images are representative of N = 5 taken at 20x with 100 μm scale bars bottom right.

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques: Isolation, Positive Control, Marker, Staining

    Piezo1 agonist Yoda1 induced relaxation of the longitudinal strip of thoracic aorta with endothelium in mouse (A) but not rat (B). (A,B) +E = Endothelium intact (ACh caused >40% PE relaxation), PVAT removed, thoracic aorta in strips. Points shown as mean±SEM for N reported. Star is significance from two-way ANOVA p < 0.05.

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: Piezo1 agonist Yoda1 induced relaxation of the longitudinal strip of thoracic aorta with endothelium in mouse (A) but not rat (B). (A,B) +E = Endothelium intact (ACh caused >40% PE relaxation), PVAT removed, thoracic aorta in strips. Points shown as mean±SEM for N reported. Star is significance from two-way ANOVA p < 0.05.

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques: Stripping Membranes

    (A) Isometric tracing for Yoda1 CRC representing (B) quantification of Piezo1 agonist Yoda1 in rat thoracic aorta rings without PVAT, with and without endothelium (+E [78.22 ± 1.63% relaxation to ACh], −E [0.25 ± 0.57% relaxation to ACh], filled, open respectively) and without potassium (−K, circles) and with potassium (+K, triangles). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B) Symbols represent mean±SEM for N reported.

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: (A) Isometric tracing for Yoda1 CRC representing (B) quantification of Piezo1 agonist Yoda1 in rat thoracic aorta rings without PVAT, with and without endothelium (+E [78.22 ± 1.63% relaxation to ACh], −E [0.25 ± 0.57% relaxation to ACh], filled, open respectively) and without potassium (−K, circles) and with potassium (+K, triangles). (A) Arrows indicate each cumulative step with PE maximal contraction at the end. (B) Symbols represent mean±SEM for N reported.

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques:

    Effect of vehicle (DMSO, black) or Piezo1 agonist Yoda1 (grey) on rat superior mesenteric artery contraction (A) or relaxation (B; half-maximally contracted with PE) with or without PVAT (filled or open symbols, respectively). Points represent mean±SEM for N reported.

    Journal: Pharmacological research

    Article Title: Identification of Piezo1 channels in perivascular adipose tissue (PVAT) and their potential role in vascular function

    doi: 10.1016/j.phrs.2021.105995

    Figure Lengend Snippet: Effect of vehicle (DMSO, black) or Piezo1 agonist Yoda1 (grey) on rat superior mesenteric artery contraction (A) or relaxation (B; half-maximally contracted with PE) with or without PVAT (filled or open symbols, respectively). Points represent mean±SEM for N reported.

    Article Snippet: Taq-Man PCR primers were purchased from ThermoFisher Scientific (Piezo-type mechanosensitive ion channel component 1 ( Piezo1 ), catalog # Rn01432593_m1; Piezo-type mechanosensitive ion channel component 2 ( Piezo2 ), catalog # Rn01491821_m1; transient receptor potential cation channel, subfamily V, member 4 ( TRPV4 ), catalog # Rn00576745_m1; anoctamin 1, calcium activated chloride channel ( TMEM16A/ANO1 ), catalog # Rn01474520_m1; Pannexin 1 ( Panx1 ), catalog # Rn01447976_m1; actin, beta ( Actb ), catalog # Rn00667869_m1).

    Techniques: